Characterization of two alternative promoters for integrase expression in the clc genomic island of Pseudomonas sp. strain B13
نویسندگان
چکیده
منابع مشابه
Unusual integrase gene expression on the clc genomic island in Pseudomonas sp. strain B13.
An unusual type of gene expression from an integrase promoter was found in cultures of the bacterium Pseudomonas sp. strain B13. The promoter controls expression of the intB13 integrase gene, which is present near the right end of a 105-kb conjugative genomic island (the clc element) encoding catabolism of aromatic compounds. The enzymatic activity of integrase IntB13 is essential for site-spec...
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Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105-kilobase clc element of Pseudomonas sp. Strain B13.
Pseudomonas sp. strain B13 carries the clcRABDE genes encoding chlorocatechol-degradative enzymes on the self-transmissible 105-kb clc element. The element integrates site and orientation specifically into the chromosomes of various bacterial recipients, with a glycine tRNA structural gene (glyV) as the integration site. We report here the localization and nucleotide sequence of the integrase g...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2003
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2003.03548.x